Denitrification and n-alkane metabolism by Pseudomonas aeruginosa. by Helen Mary Swain

Cover of: Denitrification and n-alkane metabolism by Pseudomonas aeruginosa. | Helen Mary Swain

Published by Univ. of Birmingham in Birmingham .

Written in English

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Thesis (Ph.D.) - Univ. of Birmingham, Dept of Biochemistry.

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Open LibraryOL21621078M

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Pseudomonas aeruginosa strain can grow aerobically with various n-alkanes as the sole source of carbon and energy. Anaerobic growth, with nitrate as the Denitrification and n-alkane metabolism by Pseudomonas aeruginosa.

book electron acceptor, occurs with. All these processes are mediated by fine-tuned stable and transient protein-protein interactions. Recently, an interactomic approach was used to determine the exact protein-protein interactions involved in the Denitrification and n-alkane metabolism by Pseudomonas aeruginosa.

book of the denitrification apparatus of Pseudomonas by:   Suspensions of Pseudomonas aeruginosa and Bacillus cereus were continuously sparged with nitrogen to remove gaseous products of nitrate reduction.

Under these conditions, P. aeruginosa denitrified nitrate to 4% NO, 47% N 2 O and 49% N 2. cereus reduced nitrate to 94% nitrite, 2% NO and 5% N 2 O. cereus was unable to reduce NO or N 2 O as sole electron acceptor, whereas P. aeruginosa Cited by: Denitrification ofnitrate.

Measurements were made during anaerobic growth on nitrate to compare the denitrification properties ofPseu-domonas stutzeri, Pseudomonas aeruginosa, andParacoccus denitrificans. Cell yield reflect-ed the net energy gain, and the amount and composition ofthe gases evolved reflected the relative rates ofintermediate Cited by: In cystic fibrosis airway infection, Pseudomonas aeruginosa forms a microaerobic biofilm and undergoes significant physiological changes.

It is important to understand the bacteriumapos;s metabolism at microaerobic conditions. In this work, the culture properties and two indicators (the denitrification‐accepted e ‐ fraction and an NAD(P)H fluorescence fraction) for the cultureapos;s Cited by: 5.

Bryan, B.,“Cell Yield and Energy Characteristics of Denitrification with Pseudomonas stutzeri and Pseudomonas aeruginosa,” Ph.D. Thesis, Univ. Using a pure culture of Pseudomonas fluorescens as a model system nitrite inhibition of denitrification was studies.

A mineral media with acetate and nitrate as sole electron donor and acceptor, respectively, was used. Results obtained in continuous stirred‐tank reactors (CSTR) operated at pH values between and showed that growth inhibition depended only on the nitrite undissociated.

Poster Design & Printing by Genigraphics® - A Mathematical Model of Denitrification in Pseudomonas aeruginosa Seda Arat1, Michael Schlais2, George Bullerjahn2, Reinhard Laubenbacher1 1Department of Mathematics & Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 2Department of Biological Sciences, Bowling Green State University, Bowling Green, OH.

Pseudomonas sp. C27 can effectively conduct mixotrophic denitrifying sulfide removal (DSR) reactions using both organic matters and sulfide as electron donors. This study conducted DSR tests using C27 and quantitatively analyzed the protein abundances at C/N =and At C/N =C27 principally adopted autotrophic denitrification pathway in DSR reaction.

Free nitrous acid (FNA) has recently been demonstrated as an antimicrobial agent on a range of micro-organisms, especially in wastewater-treatment systems. However, the antimicrobial mechanism of FNA is largely unknown.

Here, we report that the antimicrobial effects of FNA are multitargeted. The response of a model denitrifier, Pseudomnas aeruginosa PAO1 (PAO1), common in wastewater treatment. Pseudomonas aeruginosa: A Model for Biofilm Formation Diane McDougald University of New South Wales, School of Biotechnology and Biomolecular Science and Center for Marine Biofouling and Bio‐Innovation, Sydney, NSWAustralia.

Continuous cultures of Pseudomonas aeruginosa (ATCC ) maintained at different dissolved oxygen concentrations (DO) were studied for the effects of DO on various culture properties, especially aerobic respiration and denitrification.

The DO was varied from 0 mg/liter (completely anoxic conditions) to mg/liter and measured with optical sensors that could accurately determine very low DO. Herein, Pseudomonas stutzeri was used to explore the effects of AgNPs on denitrification and cytotoxicity.

The denitrification efficiency decreased from % in the AgNP-free treatment to %, % and % with treatments. Chronic Pseudomonas aeruginosa lung infection is the most severe complication in patients with cystic fibrosis (CF).

The infection is characterized by the formation of biofilm surrounded by numerous polymorphonuclear leukocytes (PMNs) and strong O2 depletion in the endobronchial mucus. We have reported that O2 is mainly consumed by the activated PMNs, while O2 consumption by aerobic.

In cystic fibrosis airway infection, Pseudomonas aeruginosa forms a microaerobic biofilm and undergoes significant physiological changes.

It is important to understand the bacteriumapos;s metabolism. Pseudomonas was defined as heterotrophic and the aerobic growth rate was much higher than the anoxic growth rate (Koike & Hattori ).

Although some species of Pseudomonas such as Pseudomonas stutzeri and Pseudomonas aeruginosa had the ability to denitrify NO 3-N, the anoxic condition of no oxygen was necessary (Carlson & Ingraham ).

Pseudomonas aeruginosa is a prominent gram-negative human pathogen that can thrive in a variety of challenging infection sites. Comprehending this remarkable feat is central to developing strategies to combat infection.

• Many of the adaptations that occur in Pseudomonas aeruginosa during infection compared to standard laboratory conditions are centered on core metabolism. Chronic Pseudomonas aeruginosa lung infection is the most severe complication in patients with cystic fibrosis (CF).

The infection is characterized by the formation of biofilm surrounded by numerous polymorphonuclear leukocytes (PMNs) and strong O 2 depletion in the endobronchial mucus. We have reported that O 2 is mainly consumed by the activated PMNs, while O 2 consumption by aerobic. A comparison was made of denitrification by Pseudomonas stutzeri, Pseudomonas aeruginosa, and Paracoccus denitrificans.

Although all three organisms reduced nitrate to dinitrogen gas, they did so. A comparison was made of denitrification by Pseudomonas stutzeri, Pseudomonas aeruginosa, and Paracoccus denitrificans. Although all three organisms reduced nitrate to dinitrogen gas, they did so at different rates and accumulated different kinds and amounts of intermediates.

Their rates of anaerobic growth on nitrate varied about fold; concomitant gas production varied more than 8-fold. Optimal cell yield of Pseudomonas aeruginosa grown under denitrifying conditions was obtained with mM nitrate as the terminal electron acceptor, irrespective of the medium used.

An aerobic denitrification strain, Pseudomonas balearica RAD, was identified and showed efficient inorganic nitrogen removal ability. The average NO3−-N, NO2−-N, and total ammonium nitrogen (TAN) removal rate (>95% removal efficiency) in a batch test was mg/(L∙h), mg/(L∙h), and mg/(L∙h), respectively.

Meanwhile, optimal incubate conditions were obtained. A two-step denitrification model was used to fit the transformation of nitrogen species when strain T13 used NH 4 +, NO 2 – or NO 3 – as sole nitrogen sources by using AQUASIM to test the validity of stoichiometric coefficients and kinetic parameters in Table fitting results were shown in Fig.

2, Fig. 3, Fig. 4, Fig. er, the transformations of nitrogen species by strain T In contrast to most denitrifiers studied so far, Pseudomonas stutzeri TR2 produces low levels of nitrous oxide (N2O) even under aerobic conditions.

We compared the denitrification activity of strain TR2 with those of various denitrifiers in an artificial medium that was derived from piggery wastewater. Strain TR2 exhibited strong denitrification activity and produced little N2O under all.

aeruginosa has a flexible metabolism that can utilise nitric oxides as alternative electron acceptors to produce energy when oxygen is depleted. This process is called denitrification and is also performed by many other bacteria.

The stepwise process of denitrification in P. aeruginosa is as follows: NO 3 − → NO 2 − → NO → N 2 O. In Pseudomonas aeruginosa, a ubiquitous gram-negative environmental bacterium, denitrifying genes are also regulated by N-oxides and oxygen levels through a regulatory network requiring ANR, DNR regulatory proteins, and a nitrate-responding two-component regulator, NarXL (45).

Aerobic denitrification ability has been reported in some bacteria such as Thiosphaera pantotropha (Robertson and Kuenen, ), Alcaligenes faecalis (Joo et al., ), Pseudomonas aeruginosa. The interference of toxic heavy metals in the process of microbial aerobic denitrification is a hot issue in industry wastewater treatment in recent years.

In this study, a multifunctional aerobic denitrifying bacterium - Pseudomonas aeruginosa G12 isolated from sewage sludge was used to explore the. Studies on two potential isolates 10 and TMR, isolated from mangrove and sand dune sediments of Goa and identified as Pseudomonas nitroreducens and Pseudomonas aeruginosa, respectively, elucidate the various adaptive mechanisms undertaken by denitrifying bacteria in response to hydrocarbons.

Hydrocarbons have a prominent influence on. Pseudomonas putida KT contains a branched aerobic respiratory chain with multiple terminal oxidases. Their relative proportion varies according to environmental conditions.

The role of the oxygen‐responsive ANR global regulator on expression of these terminal oxidases was analysed. Abstract. Pseudomonas aeruginosa is able to grow under anoxic conditions in the presence of nitrate or nitrite. This process is known as denitrification.

Denitrification is not only important in bacterial ecology but also many studies indicate that it is associated with P. aeruginosa infection. While the regulation of denitrification is well studied, how the cells behave under denitrifying. Key Points. Denitrification generally proceeds through a stepwise reduction of some combination of the following intermediate forms: NO 3 − → NO 2 − → NO + N 2 O → N 2.; Generally, several species of bacteria are involved in the complete reduction of nitrate to molecular nitrogen, and more than one enzymatic pathway has been identified in the reduction process.

The book edited by Professor P. Cornelis on molecular approaches to Pseudomonas is an excellent source of information on the biology of P. aeruginosa PAO and P. putida KT The P. fluorescens strains have not received much attention and perhaps nobody can predict how long the eclipse will last.

Pseudomonas aeruginosa san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to mM of cadmium. Leaving biomass of P.

aeruginosa san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of. Despite its restriction to a relatively small number of procaryotes, denitrification exerts a pervasive effect on the metabolism of organisms at many levels of biology.

In the development of their Gaia, or Mother Earth, hypothesis of the Earth’s atmosphere as a circulatory system of biological origin, Margulis and Lovelock () attributed a.

Pseudomonas: Volume 3 Biosynthesis of Macromolecules and Molecular Metabolism Author: Juan-Luis Ramos Published by Springer US ISBN: DOI: / Table of Contents: Lipopolysaccharides of Pseudomonas aeruginosa Alginate Biosynthesis.

Pseudomonas are aerobic bacteria, but in some cases they can use nitrate as alternate electron acceptor and carry out denitrification (P.

aeruginosa, P. stutzeri, and some P. fluorescens biovars), reducing nitrate to N 2 O or N 2. Additionally, P. chloritidismutans can utilize chlorate (ClO 3 –) as an alternative energy-yielding electron. Pseudomonas putida was found to degrade n-alkanes, 4 P. aeruginosa W10 preferentially utilized n-C 16, but also degraded naphthalene, phenanthrene, fluoranthene and pyrene, 5 Pseudomonas F was found to utilize fluorene, 6 Pseudomonas NCIB had the ability to degrade fluorene, dibenzofuran and dibenzothiophene, 7 and P.

aeruginosa DQ8. Interdependence of respiratory NO reduction and nitrite reduction revealed by mutagenesis of nirQ, a novel gene in the denitrification gene cluster of Pseudomonas stutzeri. FEBS Lett. Dec 21; (3)– Kalkowski I, Conrad R. Metabolism of nitric oxide in denitrifying Pseudomonas aeruginosa and nitrate-respiring Bacillus cereus.

Results of a typical experiment to examine n-hexadecane metabolism by P. aeruginosa under sequential aerobic and anaerobic denitrifying conditions.

CHAYABUTRA AND JU. Enter search terms. Keep search filters New search. Advanced search.To remove nitrate in wastewater treatment plant effluent, an aerobic denitrifier was newly isolated from the surface flow constructed wetland and identified as Pseudomonas mendocina strain GL6. It exhibited efficient aerobic denitrification ability, with the nitrate removal rate of mg (N)L−1h−1.

Sequence amplification indicated that the denitrification genes napA, nirK, norB, and.An illustration of an open book. Books. An illustration of two cells of a film strip. Video. An illustration of an audio speaker. Audio An illustration of a " floppy disk.

Nitrous Oxide Production in Sputum from Cystic Fibrosis Patients with Chronic Pseudomonas aeruginosa Lung Infection.

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